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产品简介
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真正的快速解决方案 |
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The iQ-Check system takes full advantage of real-time
PCR to provide a faster reliable solution in food pathogen
testing. A complete range of kits are available for
qualitative detection of major food pathogens in all
food and environmental samples. Due to the high sensitivity
and specificity of real-time PCR, a single overnight
enrichment is sufficient to obtain results equivalent
to or better than ISO or other reference methods. |
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iQ-Check的原理 |
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Genes targeted are specific: for example, the gene
involved in the invasive bacterial process (iagA) for
detecting Salmonella spp. and the gene involved in the
listeriolysine virulence process (hlyA) for detecting
Listeria monocytogenes. Detection of the fluorescent
signal of these amplified sequences is an indication
of the presence of the pathogen in the sample being
tested. In a multiplex assay, each PCR well has an internal
control DNA, which is amplified at the same time as
the target DNA, but detected by a different probe and
fluorophore. |
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Maximum Flexibility |
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Developed as a flexible, open system, the iQ-Check
kits can be used with different Bio-Rad real-time PCR
instruments. A single sample or as many as 94 samples
can be run on either smaller instruments or higher throughput
instruments, all to fit a laboratory's needs and throughput.
Both a 96 and 48-well instrument are available, with
the ability to run up to 4 instruments from a single
computer at the same time. |
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Optimized Cost and Work Flow |
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With a time-to-result of 24 hrs or less, it is now
possible to optimize cost and reactivity, improving
HACCP procedures and overall productivity. Innovative
media, reagents and leading technology provide a complete
solution for the most efficient control of food products
and the industrial environment. |
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iQ-Check系统采用实时荧光定量PCR方法所有的的优点，为水中病原微生物提供快速和可靠地检测解决方案。iQ-Check实时荧光定量PCR试剂盒为所有环境水中的军团菌(Legionella)和嗜肺型军团菌(Legionella
Pneumophila)定性和定量检测试剂盒。 |
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iQ-Check实时荧光定量PCR试剂盒是基于基因学方法用于食品病原微生物和水中病原微生物检测的突破，iQ-Check实时荧光定量PCR试剂盒采用聚合酶链式反应(PCR)和实时检测和定量技术相结合，iQ-Check军团菌实时荧光定量PCR试剂盒包括：DNA引物和特异生物的荧光分子探针。如果水样中存在军团菌和嗜肺型军团菌，DNA得以扩增和荧光强度增加，其被iCycler
iQ系统实时检测和记录。在每个反应管内存在的内标通过检测抑制因子的存在和阴性结果可靠性的确认来评估每个扩增反应的效率。每个反应包含有两种荧光染料，用作目标DNA和内标的标记探针，包括一套定量标准的以保证军团菌和嗜肺型军团菌的实时定量检测(推荐采用Chromo4型四色实时荧光定量PCR仪)。 |
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iQ-Check的特点
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Faster Results |
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Test results within hours after overnight enrichment |
| Colony confirmation with validated chromogenic agar |
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Easy To Use |
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Simple DNA extraction |
| Automated amplification and detection |
| Run multiple pathogen tests at the same time |
| Ready-to-use reagents, including extraction reagents |
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Minimal Risk |
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Positive, negative and internal PCR inhibition controls |
| Specific primers and probles |
| No post-PCR steps for minimal contamination risk |
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A Complete Solution |
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Standardized Bio-Rad reagents: |
| - Enrichment media |
| - Real-time PCR iQ-Check kits |
| - Chromogenic agars |
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Validated Bio-Rad equipment and software: |
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- Real-time PCR detection systems |
| - Automated data interpretation and traceability |
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iQ-Check试剂盒产品信息
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产品编号 |
产品描述 |
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78123 |
Kit for the detection of Salmonella spp.
iQ-Check沙门氏菌属检测试剂盒，96个测试/盒 |
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78124 |
Kit for the detection of Listeria monocytogenes
iQ-Check单增李斯特菌检测试剂盒，96个测试/盒 |
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78113 |
Kit for the detection of Listeria spp.
iQ-Check李斯特菌属检测试剂盒，96个测试/盒 |
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78114 |
Kit for the detection of E.coli O157:H7
iQ-Check大肠杆菌O157:H7检测试剂盒，96个测试/盒 |
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