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RAPID'水中大肠菌群快速培养基检测
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RAPID'E.coli 2 Medium for Detection and Enumeration
of E.coli and Coliforms |
Approved by AFNOR according to ISO 16140 for
E. coli and other coliforms at 37℃ and E. coli at
44℃.
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RAPID'E.coli 2 is also AOAC-RI (Certificate
#050601) and Nordval approved.
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Intended Use
RAPID'E.coli 2 is a selective chromogenic medium
used for direct enumeration (without colony confirmation)
of E. coli and other coliforms in food products
for human and animal consumption. RAPID'E.coli 2
is not available for water testing in the USA. |
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RAPID'水中大肠菌群快速培养基检测试剂盒的原理
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The principle of the medium is based on
simultaneous detection of two enzymatic activities:
• One substrate specific to β-D-galactosidase (GAL) that
induces blue coloration of colonies positive for this enzyme
• One substrate specific to β-D-glucuronidase (GLUC) that
induces pink coloration of colonies positive for this enzyme.
Coliforms (GAL+/GLUC) form blue to green colonies; E. coli
(GAL+/GLUC+) form violet to pink colonies.
GLUC detection gives the culture medium a high specificity.
E. coli is one of the only species of enterobacteria to
possess this enzyme. |
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Note: β-D-glucuronidase-negative E. coli
strains exist (approximately 34%), such as E. coli O157.
Some Salmonella serovars and a few Shigella species also
contain β-D-glucuronidase (<1.5%). |
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RAPID'水中大肠菌群快速培养基检测试剂盒的特点
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Single medium for direct enumeration of
both E. coli and coliforms on the same plate |
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Complete results in 18 - 24 hr |
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Gas production not necessary for differentiation of
colonies |
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Easy-to-read chromogenic reaction |
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Economical and easy to use |
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RAPID'水中大肠菌群快速培养基检测实验方法
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The figure below provides an overview of
the protocol for the enumeration of E. coli and
coliforms with RAPID'E.coli 2 medium: |
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RAPID'水中大肠菌群快速培养基检测实验所需试剂
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产品编号 |
产品描述 |
| 355-5299 |
RAPID'E.coli 2 Agar, ready-to-use, 6 x 100 ml bottles |
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356-4024 |
RAPID'E.coli 2 Agar, dehydrated, 500 g |
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在线产品样本(PDF格式) |
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