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病原微生物的检测

李斯特菌的检测
沙门氏菌的检测
大肠杆菌O157检测
弯曲杆菌的检测
军团菌的检测
大肠杆菌的检测
肠球菌的检测
葡萄球菌的检测
狂犬病的检测

其它相关检测

转基因食品的检测
猪旋毛虫的检测

伯乐相关检测仪器

RT-PCR基础简介
伯乐系列PCR仪
伯乐系列RT-PCR仪
伯乐其它检测仪器

伯乐实验室消耗品

伯乐实验室消耗品
 

PCR基础简介

Backgrand of PCR Technologies

Real time PCR

 

Real-Time PCR Process

  In real-time PCR, the detection step is combined with amplification: the amplified target nucleic acid is visualized in real time as a fluorescent signal. This signal is generated at each PCR cycle only when a specific oligonucleotide probe called a molecular beacon, linked to a fluorophore, hybridizes with the amplified target DNA. As the amount of amplified DNA increases with each round of amplification, fluorescence intensity also increases. During each PCR cycle, a real-time PCR instrument such as the iQ5 or Chromo4 system measures the fluorescence signal, and the associated software plots a graph of the fluorescence intensity versus the number of cycles.
 
 
Real-Time PCR Quantification
 
  Real-time PCR is the perfect method for the quantification of a target nucleic acid in a food or water sample. After amplification of the target nucleic acid in parallel to calibrated quantification standards, the number of initial DNA copies in the sample can be automatically calculated by the real-time software without requiring any additional operations. The software simply constructs a standard curve from the values recorded for the quantification standards and plots the values for the samples against this standard curve.
   
上次更新时间:2010年06月07日
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