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GMO Analysis
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According to the European regulations
on genetically modified (GM) foods, a product is subject
to mandatory labeling if it consists of, or is derived
from genetically modified organisms (GMOs). The threshold
for labeling is 0.9% of each ingredient for EU-authorized
GMOs. This threshold is 0.5% for GMOs not authorized
for marketing in Europe but having successfully passed
all safety assessments. The new regulations relate to
food, feed, flavorings, and additives and require full
traceability systems throughout the entire production
chain. Accurate and high-performance tools are required
for the quantification of the GM material contained
in all the different matrices from the seed to the final
product. Real-time PCR is the gold standard used for
GMO detection and quantification in foods. |
| Most of the GM crops currently available
are maize and soya. They account for more than 80% of
the total GMO-planted area worldwide. About 30,000 ingredients
today contain maize and soya in their formulation and
are affected by GMO traceability and labeling. |
| Bio-Rad iQ-Check GMO kits provide a
simple decision-making solution for manufacturers concerned
by maize and soya ingredient labeling. iQ-Check GMO
are ready-to-use kits based on real-time PCR. The kits
enable reliable and accurate detection and quantification
of the level of GMO presence in each sample: from raw
material to processed food. |
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iQ-Check GMO Detection and Quantification Principle
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According to European regulations, the
genetically modified organism (GMO) content is determined
as a percentage of the GM DNA present in the sample
and the total maize or soya DNA content. |
| The GM DNA is detected by the screening
of a special sequence, the promoter p35S, present in
most transformation events in GM crops. |
| iQ-Check screen GMO p35S/tNos kit detects,
in parallel with the p35S sequence, a terminal sequence,
tNos, present in most unauthorized GMOs on the market. |
| GM DNA is always detected and quantified
in the presence of an artificial sequence called the
internal control. This sequence is included in the amplification
mix provided in the kit and is used to assess the efficiency
of the amplification reaction. |
| Total maize and soya DNA content is
determined by the presence of a specific plant reference
gene. |
| The quantification standards included
in the iQ-Check GMO quantification kits enable precise
determination of the GM DNA/total maize or soya DNA
ratio. |
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iQ-Check GMO Detection and Quantification Principle
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Ready-to-use reagents for maximum reproducibility |
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Reliable detection of GMO presence |
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Accurate quantification of 20–20,000 DNA copies |
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No post-PCR operations |
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No post-PCR contamination risk |
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Minimal hands-on time |
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Flexibility to use different thermal cyclers |
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iQ-Check GMO Kit Contents
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iQ-Check Kits for Detection and Quantification
of the Level of GMOs
The kits contain all the reagents required to perform
real-time PCR analysis of the sample:
• Fluorescent probes
• Amplification mix containing Taq DNA polymerase, primers,
and internal inhibition control
• Positive and negative controls
• Ready-to-use quantification standards in all GMO quantification
kits
Reagents are provided in a ready-to-use format for 96
PCR reactions. |
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iQ-Check GMO Kits
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产品编号 |
产品描述 |
| EasyPrep Extraction Apparatus |
| 357-8111 |
EasyPrep DNA Extraction System, dual-unit apparatus
for genomic DNA extraction and purification |
| iPure GMO Extraction Kit |
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357-8112 |
iPure GMO DNA Extraction Kit, ready-to-use reagent
kit for genomic DNA extraction and purification,
48 tests |
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GMO Extraction System
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A reliable genetically modified organism (GMO) testing
result is highly dependent on the quality of the DNA
extracted from a sample for analysis by real-time PCR.
Food and plant samples are extremely heterogeneous;
they contain many different compounds, such as fats
and polysaccharides, which can lead to suboptimal DNA
extraction and processing. |
| The Bio-Rad DNA extraction system consistently
provides the user with high-quality DNA, free of any
material that could interfere with downstream applications
such as PCR, and is specifically suited to the treatment
of GMO-related matrices. |
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EasyPrep Extraction Apparatus
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The EasyPrep apparatus represents a technology that
responds to a rapid and standardized preparation of
nucleic acids while remaining truly simple to operate.
The system combines positive-pressure processing with
advanced membrane devices, adapted media, and intelligent
chemistry. |
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iPure GMO Extraction Kit
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Suitable for use with the EasyPrep apparatus, the
iPure GMO extraction kit allows the rapid availability
of PCR-ready DNA for GMO detection. The kit is suitable
for extraction from all kinds of samples to be analyzed. |
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iPure GMO Extraction Kit
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Universal: one protocol for all matrices |
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Extraction of up to 1 g of sample |
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Reproducible high DNA quality for reliable PCR |
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Superior efficiency with complex samples |
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24 samples in parallel |
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Ready-to-use reagents |
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2.5-3 hour protocol |
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A single centrifugation step |
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Contents of the GMO Extraction System Kit
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Each kit contains all the reagents necessary
for 48 extractions:
• Anion exchange resin
• Solubilization, lysis,
coating, wash, and elution buffers
• Extraction columns
• Isopropanol
• Ethanol 70% |
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Bulletin_2697_GMO Testing.pdf |
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在线产品样本(PDF格式) |
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