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耶拿 - 聚丙烯酰胺凝胶电泳系统
analytikjena Polyacrylamide Gel Electrophoresis (PAGE)
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Mini-V8.10型聚丙烯酰胺凝胶垂直电泳仪
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Designed for the separation and blotting of analytical and semi-preparative quantities of proteins in vertical 8.7 x 7.2 cm polyacrylamide (PAGE) gels.
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- Runs 1 or 2 gels simultaneously
- Patented drop-in wedge automatically positions gels without the use of gaskets or clamps
- Electroblotting performed in the same buffer tank
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The Mini-V system is an integrated system for rapid, high-quality separation and blotting of proteins (and nucleic acids) in a vertical mini-gel format. Its two primary components, the Mini-V 8.10 Vertical Gel electrophoresis Apparatus and the Mini-V 8.10 Blot Module, are supplemented by a complete selection of accessories and replacement parts, including an external gel casting system.
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·专利楔形卡槽可以容易地固定凝胶，不需额外的垫或夹子
·可同时电泳一或两块8.7x7.2cm胶，最多同时电泳20个样品
·不同高度和厚度的胶均可电泳
·可与绝大多数8X10cm预制胶相配
·可制备三种不同厚度凝胶：0.5，0.75，1.5mm
·透明外壳便于观察电泳状况
·带电线安全盖
·可选：Mini-V8·10制胶夹，Mini-V8·10印迹转移模块和Mini-V8·10制胶系统(可同时制2至9块胶) |
The Mini-V8.10 is designed for separation and blotting in a vertical 8.7 x 7.2 (W x L) cm mini-gel format. The patented drop-in wedge automatically positions gels without the use of gaskets or clamps. Additional gels of different length and thickness can be electrophoresed. Most commercial available pre-cast 8 x 10 cm format PAGE gels can be used with the system. Up to 20 samples can be separated by running 2 gels simultaneously. 3 sets of spacers with different thickness (0.5; 0.75 and 1.5 mm) allow optimal adaptation of gel thickness for various applications.
The Mini-V8.10 Blot Module operates in the electrophoresis tank of the Mini-V8.10 for the transfer of proteins (and nucleic acids) to membranes. 1 or 2 gels can be blotted simultaneously. The 1000 ml Puffer volume takes care for an efficient cooling even at higher current.
It is recommended to use the Mini-V8.10 Gel Casting Clamp for simple, leak-free casting of gels. This allows casting of single gels in a fraction of the time of the "conventional" tape or grease and binder clip methods.
The Mini-V8.10 Gel Casting System is designed for easy set-up and cleaning and allows to cast 2 to 9 gels simultaneously for increased reproducibility. |
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Mini-V8.10型聚丙烯酰胺凝胶垂直电泳仪的技术参数
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| 产品型号 |
Mini-V8.10型 |
| 设备规格 |
16.7(宽) x 14.0长) x 15.0(深) cm |
| 凝胶规格 |
8.7(宽) x 7.2(长) cm |
| 玻璃板规格 |
10.25(宽) x 7.25/8.25(长) cm |
| 凝胶数量 |
1 或 2 |
| 样品容量 |
最多10个样品(样品总数20个) |
| 样品体积 |
12 - 475 µl (决定于凝胶的厚度) |
| 梳子规格 |
梳子厚度(mm) |
孔数 |
孔宽(mm) |
容量/孔(µl)a |
| 0.75 |
6 |
10.7 |
40 |
| 1.0 |
10 |
5.6 |
20 |
| 1.5 |
1 prep. + 2 marker lanes |
63.4 |
475c |
| 1.5 |
6 |
10.7 |
80 |
| 1.5 |
10 |
5.6 |
40 |
| 缓冲液体积 |
600 - 650 ml (total buffer volume) for electrophoresis 1,000 ml for blotting |
a Volume calculated for an insertion depth of 5 mm
c Volume of the central preparative slot
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Mini-V8.10型聚丙烯酰胺凝胶垂直电泳仪的订货信息
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产品编号
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产品描述
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| 21078050 |
Mini-V 8.10 Gel Electrophoresis System (EU version), complete system with Mini-V8.10 Gel Electrophoresis Apparatus and Mini-V8.10 Blot Module
(Note! The Mini-V8.10 Gel Casting Clamp is sold separately) |
| 21078043 |
Mini-V 8.10 Gel Electrophoresis Apparatus (EU version), complete with buffer tank, safety lid, gel support tray, gel wedge block, 2 pairs of glass plates (2 short glass plates 7.25 x 10.25 cm and 2 long glass plates 8.25 x 10.25 cm), 2 sets of 0.75 mm thick side spacers, 2 x 10 well combs (0.75 mm thick) and manual
(Note! The Mini-V8.10 Gel Casting Clamp is sold separately.) |
| 21078019 |
Mini-V 8.10 Blot Module, complete with blot restrainer, blot support frame, clamping knob, three transfer pressure pads and manual. |
| 21078027 |
Mini-V 8.10 Gel Casting System, complete with a casting chamber with threaded posts, an end plate with silicone gasket and fill port, displacement blocks (1 x 1 mm, 2 mm and 5 mm and 5 x 9 mm), 20 gel separation sheets, 10 pairs of glass plates, wing nuts and cap nuts, stopcock, tubing connector, stopper and manual
(Note! Combs and spacers are sold separately!) |
| Accessories: |
| 21078241 |
Mini-V 8.10 Gel Casting Clamp (1 / pkg) |
| 21078233 |
Mini-V 8.10 Gel Casting Clamp (2 / pkg) |
| 21078126 |
Mini-V 8.10 Casting Stand (acrylic casting stand) |
| 21078035 |
Glass Plates (mixed pairs, 8.25 x 10.25 cm / 7.25 x 10.25 cm) (10 pairs) |
| 21078076 |
Gel Loading Template (4 / pkg) |
| 11098019 |
Spring Clips (12 / pkg) |
| 21105333 |
Stainless Steel Binder Clips (12 / pkg) |
| Spacer Sets (side spacers): |
| 11958147 |
0.75 mm (2 pairs) |
| 11958154 |
1.5 mm (2 pairs) |
| Combs, 0.75 mm (max. volume / well)*: |
| 11958089 |
6 wells (40 µl) |
| 11958113 |
10 wells (20 µl) |
| Combs, 1.5 mm (max. volume / well)*: |
| 11958097 |
6 wells (80 µl) |
| 11958121 |
10 wells (40 µl) |
| 11958063 |
preparative, with 2 marker lanes (475 µl)*** |
| Replacement Parts |
| 11958162 |
Platinum/Niobium Upper Electrode Replacement Kit Mini-V |
| 11958261 |
Lower Electrode Replacement Kit Mini-V |
| 11958469 |
Banana Plug Replacement Kit (Includes 2 banana plugs, 1 red and 1 black cap nut and 2 lock washers) |
* Volumes calculated for an insertion depth of 5 mm
*** Volume of the central preparative well
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