|
 |
Biometra-TOptical实时定量PCR仪
Biometra TOptical Real-Time PCR Thermocycler
|
|
 |
 |
TOptical实时定量PCR仪 - 配置最灵活的实时定量PCR仪
|
|
- Interchangeable block modules
- Upgrade at existing TProfessional Thermocyclers possible
- Optionally gradient enabled
- 6 positions for color filter modules
- Free customisation with color filter modules
- definition of the number of filters to be implemented
- choice amongst 10 different filters (6 fluorescence, 4 FRET)
- filter combinations covering the whole range of commonly used dyes
- Patented scanner system with optical fibers
- Filter-upgrade / interchange at installed units possible
- Optimal excitation by three long lasting LEDs
- Easy to use software including commonly used analysis functions
|
 |
Filter
|
Biometra offers six different color filter mdules and five FRET filter modules for the TOptical Thermocycler. The six color filter modules cover a spectrum from green to far red emission. For each filter examples of dyes that can be detected are given. Of course also other dyes having similar excitation and emission maxima may be used. In addition, for FRET applications five filter modules and one filter module for protein stability measurements are avaible. The corresponding dye combinations are given in the table.
|
Filter
|
Wavelengths (abs./em.)
|
Dyes
|
TOptical Filter Module 1 |
470nm/520nm |
FAM, SYBR Green, Alexa488 |
TOptical Filter Module 2 |
515nm/545nm |
JOE, VIC, HEX, Yakima Yellow, TET, Atto 520 |
TOptical Filter Module 3 |
535nm/580nm |
TAMRA, DFO, Alexa 546, NED, Cy3, Atto 565, Dragon Fly Orange |
TOptical Filter Module 4 |
565nm/605nm |
ROX, TexasRed, Cy3.5, Atto 610 |
TOptical Filter Module 5 |
630nm/670nm |
Cy5, Alexa 633, Quasar 670 |
TOptical Filter Module 6 |
660nm/705nm |
LightCycler Red 705, Alexa 680 |
TOptical FRET Module 1 |
470nm/580nm |
FAM/TAMRA |
TOptical FRET Module 2 |
470nm/670nm |
FAM/Cy5 |
TOptical FRET Module 3 |
470nm/705nm |
FAM/Cy5.5 |
TOptical FRET Module 4 |
515nm/670nm |
JOE/Cy5 |
TOptical FRET Module 5 |
470nm/605nm |
FAM/ROX |
TOptical Filter Module SYPRO |
490nm/580nm |
SYPRO Orange |
|
TOptical Lightpath
|
Fluorescent light for the excitation of dyes is emitted by the long lasting LEDs. The light is transmitted by high performance optical fibers to collimator lenses. The fluorescent light is bundled and transmitted to an excitation filter of a color filter module located on a rotating filter wheel. The light is deflected by a beam splitter and transferred by optical fibers to a lens array in a shuttle that scans the sample block column by column. The light excites the fluorescent dyes in the reaction mix. The fluorescent dyes then emit light of a higher wavelength that is collected by the lenses in the shuttle system and transferred by optical fibers back to the filter color module. In the color module the light passes the beam splitter and two emission filters and is then further transferred to the channel photo multiplier for detection.
|
 |
TOptical Excitation by LED technology
|
The excitation spetra of the blue, white and red long lasting LED (dotted lines) cover very well the absorption spectra of the commonly used fluorescent dyes FAM, JOE/HEX/VIC, 6-ROX, TET, TAMRA, CY5 and Cy 5.5 (solid lines). In combination with the beam transfer by high perfomance optical fibers and the detection by the Channel Photo Multiplier (CPM) superb sensitivity is achieved.
|
 |
 |
TOptical实时定量PCR仪的技术参数
|
|
仪器名称
|
TProfessional Basic基因扩增仪 (PCR仪)
|
模块模式 |
96 well,10–80 µl |
最大加温速率* |
6.0℃ |
平均加温速率* |
5.0℃ |
最大降温速率* |
4.5℃ |
平均降温速率* |
3.5℃ |
斜率调整 |
最小0.1℃/秒 |
温度均一性 |
±0.15℃/15秒内@55℃,±0.25℃/15秒内@72℃,±0.50℃/15秒内@95℃ |
控制精度 |
±0.10℃ |
温度范围 |
3~99℃ |
梯度扩展/范围 |
40℃,3~99℃ |
温度增量 |
0.1℃/循环 |
时间增量 |
1秒/循环 |
加热顶盖 |
30~100℃,手动开盖,自动压力控制/10kg |
控制模式 |
通过PC控制 |
存储容量 |
PC上不受限制 |
仪器尺寸 |
280(W) x 380(H) x 430(D) mm/打开时:280(W) x 640(H) x 430(D) mm |
仪器重量 |
15.6kg(光学系统:7.4kg/主机:8.2kg) |
功 率 |
主机:最大480W/光学系统:25W |
运行环境 |
15~35℃,70%相对湿度,海拔高度最高2000m |
程序模式 |
表格和图形 |
|
|
Light source |
Three long living high power LEDs (blue, white, red) |
Filter |
Filterwheel with stepping motor, 6 positions for color modules |
Lightpath |
An array of 8 high performance optical fibers in a shuttle system directs LED light bundled by lenses to samples. The fluorescent dyes in the reaction mix are excited through from above through the lid of the tubes. The reflected light is focused by lenses and directed through optical fibers to a photomultiplier |
Color modules |
6 color modules for all commonly used real-time PCR dyes, 4 FRET filter combinations |
Sensitivity |
1 nmol/l FAM at 30 µl sample volume in a 96 well PCR plate |
Measuring time |
96 well plate (single measurement, 6 colors) appr. 8 sec |
Measuring range |
± 130 000 (±17 bit) |
|
|
qPCRsoft |
Control and analysis software |
Analysis methods |
Absolute quantification
Relative quantification
ΔΔCt method
Allelic discrimination
Efficiency calculation |
Export functions |
Excel, *.csv, qBASE, GenEx |
Security |
Administrator function/MIQE-compliant sample documentation |
|
 |
TOptical实时定量PCR仪的产品信息
|
|
产品编号
|
产品描述
|
846-070-500 |
TOptical Gradient 96,without Filter |
846-070-501 |
TOptical 96,without Filter |
846-070-510 |
TOptical Gradient 96 Module,without Filter |
846-070-511 |
TOptical Module 96,without Filter |
|
|
846-070-520 |
TOptical Filter Module 1,470nm/520nm |
846-070-521 |
TOptical Filter Module 2,515nm/545nm |
846-070-522 |
TOptical Filter Module 3,535nm/580nm |
846-070-523 |
TOptical Filter Module 4,565nm/605nm |
846-070-524 |
TOptical Filter Module 5,630nm/670nm |
846-070-525 |
TOptical Filter Module 6,660nm/705nm |
846-070-526 |
TOptical FRET Module 1,470nm/580nm |
846-070-527 |
TOptical FRET Module 2,470nm/670nm |
846-070-528 |
TOptical FRET Module 3,470nm/705nm |
846-070-529 |
TOptical FRET Module 4,515nm/670nm |
846-070-530 |
TOptical FRET Module 5,470nm/605nm |
846-070-531 |
TOptical Filter Module SYPRO,490nm/580nm |
|
|
846-050-259 |
96 well microplate, skirted,white,50 pcs. |
846-050-260 |
96 well microplate, skirted, black,50 pcs. |
846-050-258 |
Adhesive optical sealing foil,100 pcs. |
|
 |
Real-Time PCR Disclaimer for TOptical Thermocycler
|
|
Practice of the patented polymerase chain reaction (PCR) process requires a license. The Biometra [or appropriate trademark] TOptical Thermal Cycler is Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. This is a Licensed Real-Time Thermal Cycler under Applera‘s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim.
|
 |
在线产品样本(PDF格式) |
|
|
|