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德国耶拿-PCR标准试剂盒
Analytikjena Reagents for Standard PCR
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Here you find our product range for PCR reagents which are used when performing a standard PCR. We are offering a wide range on polymerases, ladders, dNTPs and as well RT-enzymes.
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innuTaq HOT-A DNA Polymerase
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innuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5‘ - 3‘ direction in the presence of magnesium. It also possesses a 5‘ - 3‘ polymerization-dependent exonuclease replacement activity but lacks a 3‘ - 5‘ exonuclease activity. innuTaq HOT-A DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling. |
Concentration*: 5 U/µl
Enzyme storage buffer: 20 mM Tris-HCl, 100 mM KCl, 0.1 EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% Nonidet P-40, 50% (v/v) Glycerol, pH 8.0 (25°C)
PCR Buffer: 10x Hotstart Buffer complete: 200 mM Tris-HCL, 500 mM KCl, 15 mM, MgCl2, pH8,5,
10x Hotstart Buffer without MgCl2: 200 mM Tris-HCL, 500 mM KCl, pH8,5
Mg2+ solution: MgCl2 stock solution, 25 mM
Store: at –20°C, avoid frequent thawing and freezing |
产品编号
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产品描述
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| 845-EZ-3000500 |
innuTaq HOT-A DNA Polymerase，500 units |
*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70°C.
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