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德国耶拿-PCR标准试剂盒
Analytikjena Reagents for Standard PCR
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Here you find our product range for PCR reagents which are used when performing a standard PCR. We are offering a wide range on polymerases, ladders, dNTPs and as well RT-enzymes.
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innuTaq RED DNA Polymerase
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innuTaq RED DNA Polymerase has a special formulation, which contains a non-toxic and non-hazardous red dye. The strong red color also provides an easy and quick check of reactions to which the enzyme has been added, facilitates the confirmation of complete mixing, making it very suitable for standard applications.
The reaction products are ready for direct gel loading and the dye serves as marker for electrophoresis progress monitoring. The presence of the dye has no effect to the application. Purified from an recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5`- 3` DNA polymerase activity. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C). |
Concentration*: 1 U/µl
Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% Nonidet P40, 50% Glycerol
PCR Buffer: 10x KCl reaction buffer, without MgCl2, 10x (NH4)2SO4 reaction buffer, without MgCl2
Mg2+ solution: MgCl2 stock solution, 25 mM
Store: at –20°C, avoid frequent thawing and freezing |
产品编号
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产品描述
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| 845-EZ-2000500 |
innuTaq RED DNA Polymerase，500 units |
*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70°C.
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