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如果采用rapidPCR操作，就需要特殊的rapidPCR聚合酶，德国耶拿公司生命科学部除提供最优化试剂盒外，还提供适配的聚合酶，以实现方便和可重复性的结果。
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10x SpeedAmp Optimization Buffer Kit
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RapidPCR allows PCR reactions in about 8 minutes by using the devices of the SpeedCycler family. This poses a challenge for any Taq DNA Polymerase. The SpeedAmp buffers were designed especially to optimize the function of Taq DNA Polymerases for the use of such rapidPCR thermal cyclers. The kit contains a choice of 10 different buffers to obtain the best performance for the reaction. Just select the suitable buffer for the application, which is also available separately.
For best possible optimization, the buffers are delivered with pH 8 and pH 9.
Note: The optimization buffers already contain MgCl2.Each SpeedAmp Optimization Buffer Kit contains 5 different 10x buffers pH 8 and 5 different 10x buffers pH 9.
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Simple PCR Optimization using SpeedCycler family
The amplification of an E. coli rRNA gene using SpeedAmp Optimization Buffer Kit (buffer 1-5, pH8) shows typical results of the first rapidPCR run. Thereby the fragments ampli-
fied with buffer 1 and 2 are poor in yield. The amplification with buffer 4 and 5 led to an unspecific primer binding. Consequently the buffer of choice is the buffer No. 3. Additional
lane 1 represents a negative control in comparison to the expected PCR products. |
| Store: at –20°C, avoid frequent thawing and freezing |
产品编号
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产品描述
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| 845-AK-1100100 |
10x SpeedAmp Optimization Buffer Kit, 10 x 0.10 ml |
| 845-AK-1100250 |
10x SpeedAmp Optimization Buffer Kit, 10 x 0.25 ml |
| 845-AK-1100500 |
10x SpeedAmp Optimization Buffer Kit, 10 x 0.50 ml |
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