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如果采用rapidPCR操作，就需要特殊的rapidPCR聚合酶，德国耶拿公司生命科学部除提供最优化试剂盒外，还提供适配的聚合酶，以实现方便和可重复性的结果。
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SpeedTaq HOT DNA Polymerase
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The SpeedTaq HOT DNA Polymerase is specially optimized for rapidPCR but also provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. Due to a synthesizing speed of 200 bp/sec, extension times and in return the total reaction time is reduced to a minimum. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formation at low temperatures during PCR setup. Thus it is optimal for amplifications running on rapidPCR thermal cyclers, as the SpeedCycler family from Analytik Jena | bio solutions. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5‘ - 3‘ direction in the presence of magnesium. It also possesses a 5‘ - 3‘ polymerization-dependent exonuclease replacement activity but lacks a 3‘ - 5‘ exonuclease activity. SpeedTaq HOT DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
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| Concentration*: 5 U/µl |
| Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% Nonidet P40, 50% Glycerol |
| PCR Buffer: 10x SpeedAmp Buffer (1 ml), buffer type has to be determined in advance using SpeedTaq HOT Optimization Set (Order-No.: 845-EZ-1120100); MgCl2 is already included in the selected buffer, no addition necessary |
| Store at –20°C, avoid frequent thawing and freezing |
产品编号
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产品描述
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| 845-EZ-7000500 |
SpeedTaq HOT DNA Polymerase，500 units |
* One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70°C.
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