北京吉诺思公司网站邮址
 

德国耶拿公司产品

基因扩增仪(PCR)
实时荧光定量PCR仪
电泳和转印系统
  聚酰胺凝胶电泳
琼脂糖凝胶电泳
脉冲场电泳系统
温度梯度电泳系统
手工核酸测序电泳
电泳电源系统
印迹及转印仪
凝胶电泳耗材
  硝酸纤维素膜
Whatman滤纸
凝胶成像系统
生物分析系统
核酸提取纯化系统
液体样品处理系统
振荡混合和快速匀浆
紫外可见分光光度计
MobiLab移动实验室
耶拿-试剂/耗材/配件
耶拿-分析和诊断试剂

相关产品链接

 
 

耶拿-硝酸纤维素转印膜

analytikjena Nitrocellulose Membranes

硝酸纤维素转印膜(Nitrocellulose Membranes)

  Nitrocellulose Membranes for Protein- and Nucleid Acid Blotting and Further Immobilisation Techniques
  • Unsupported, 100% pure nitrocellulose membrane (Protran) or reinforced nitrocellulose membrane (Optitran)
  • Excellent sensitivity, resolution and low background
  • Compatible with a variety of assays and detection methods
Protran-硝酸纤维素转印膜
  Nitrocellulose Membranes for Protein- and Nucleid Acid Blotting and Further Immobilisation Techniques
  • Best and easiest blocking of unspecific binding compared to all blotting membranes
  • Superior for chemiluminescence detection using horseradish peroxidase based systems
  • unsopported, 100% pure nitrocellulose membrane
Protran nitrocellulose membranes are the most frequently specified transfer media in the world for Western, Southern and Northern blotting. Additional the membrane is excellent for carbohydrate studies (Glycomics).
Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (peroxidase / luminol-based), colorimetric and fluorescent.
Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wet in water, and then in the transfer buffer. No other pre-treatment steps are necessary.
High binding capacity
The 0.20 µm pore size of the Protran (BA83) nitrocellulose membrane ensures high binding capacity of small samples below 20 kD by reducing "blow-through". The 0.45 µm pore size membrane (BA85) is ideal for larger molecular weight samples.
Low background
In addition to the high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.
Protein stability for years
A particular benefit of the proprietary Protran nitrocellulose formula is the proven excellent shelf life of proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran, which makes it the standard for protein blotting.
Optitran-硝酸纤维素转印膜
  Nitrocellulose Membranes for Protein- and Nucleid Acid Blotting and Further Immobilisation Techniques.
  • Reinforced nitrocellulose membranes
  • Allows multiple reprobings without loss of membrane integrity
  • Excellent signal-to-noise ration
The Optitran membrane consists of pure 100% nitrocellulose cast onto both sides of an inert polyester support material. The support in no way affects transfer conditions or results and gives the membrane exceptional handling characteristics, allowing it to be reprobed repeatedly.
The Optitran nitrocellulose membrane provides high sensitivity with very low non-specific binding without stringent washing and blocking conditions using standard nitrocellulose protocols.
The combination of flexibility, strength and excellent signal-to-noise ratios makes the Optitran membrane ideal, especially when procedures involve repeated stripping and reprobing.
Optitran supported nitrocellulose membranes combine sensitivity, strength and savings.

硝酸纤维素转印膜的选择指南

 

硝酸纤维素转印膜

Protran

Optitran

Membrane type Nitrocellulose, 100% pure Nitrocellulose, reinforced
Binding capacity: 80 - 90 µg/cm2 approx. 75 µg/cm2
Pore sizes: 0.45 µm / 0.20 µm 0.45 µm
Transfer methods:
- Semi-dry blotting ++ ++
- Tank blotting ++ ++
- Vacuum Blotting ++ ++
- Capillary blotting ++ ++
Immobilisation techniques:
- Drying Proteins Proteins
- UV-crosslinking DNA and RNA DNA and RNA
- Baking (80℃) DNA and RNA DNA and RNA
Detection methods:
- Colorimetric ++ ++
- Chemiluminescent * ++ ++
- Isotopic ++ ++
- Fluorescent Proteins -
Reprobing: + ++
++ = recommended
+ = limited use
- = not recommended
* Use of horseradish peroxidise based systems is recommended.

硝酸纤维素转印膜的订货信息

 

产品编号

产品描述

10 401 396 Protran-BA83, pore size 0.20 µm, 30 cm x 3m, 1 roll
10 401 196 Protran-BA85, pore size 0.45 µm, 30 cm x 3m, 1 roll
10 401 180 Protran-BA85, pore size 0.45 µm, 30 cm x 60 cm, 5 sheets/pkg
10 401 580 Protran-BA85, pore size 0.45 µm, 33 cm x 56 cm, 5 sheets/pkg
 
10 439 196 Optitran-BA-S 85, pore size 0.45 µm, 30 cm x 3 m, 1 roll
   
上次更新时间:2016年04月21日
返回页首
返 回
如有关于本站点的问题或建议,请向站点管理员发邮件
办公地址:北京市昌平区北清路1号珠江摩尔国际大厦3号楼2单元811室,邮政编码:102206
电话:(010) 51659588/(010) 59519455  传真:(010) 69731382 北京吉诺思科技发展有限公司 版权所有