|
|
 |
基因转移技术国际技术讲座
in-vivo Electroporation Seminars
|
|
 |
为推广体内局部基因转移技术、交流国际间相关技术的发展现状，拟定于2002年9月份在上海、北京和广州等三地分别举办EP技术交流会，特邀请国际著名专家日本名古屋大学村松达夫教授主讲。具体时间、地点如下。欢迎相关专业的学者和老师前来指导和与专家交流。
|
时间地点：
|
9月16日：上海第二医科大学 |
| 9月18日：北京大学医学部学术会议中心 |
| 9月20日：广州中山大学中山医学院。 |
讲座内容：
|
There are serious concerns about the use of viral vectors for in vivo gene transfer, especially gene therapy, such as oncogenic potential and high immunogenicity. Accordingly, an easy, safe, non-toxic and possibly efficient delivery means of genes to a specified target tissue has been an attractive issue for researchers. In this sense, in vivo electroporation (EP) is thought to be one of the promising approaches since : handling is simple, quick, i.e., within a matter of seconds, and easy; transfection efficiency is probably higher than any other nonviral method; immunogenicity is low; there may be no limitation in size and concentration of DNA transferred, and ; no special preparation of DNA is required. Although gene expression exerted is transient when transfected by in vivo EP, foreign gene expression may last as long as more than 1 year.
In EP procedures, tissues of living animals are subjected to electric field that causes not only membrane pores but also shape deformation of cells, leading to the induced membrane permeability, hence transport of large molecules such as DNA across the cell membrane. To my best knowledge, no extensive and systemic analyses of EP parameters have been conducted so far, and therefore, most experimental conditions have been empirically known. Nevertheless, the results obtained are implicative that in vivo gene EP could be applied to a wide variety of experimental and therapeutic purposes. In this brief talk, some examples are presented in relation to gene therapy, growth promotion, pharmaceutical protein production and embryo manipulation. |
联系方式：
|
北京：电话：(10) 51659588 联系人：王征星 |
| 上海：电话：(21) 62891803 联系人：沈建英 |
| 广州：电话：(20) 83229830 联系人：杨晓新 |
|
|
