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实验室超滤-Vivapure离子交换蛋白纯化浓缩器产品

Vivascience Vivapure Ion Exchange System for Protein Purifications

Vivapure离子交换膜蛋白纯化浓缩器

从混合物中分离和纯化蛋白质，是生物制药和其他生物工程研究的重要步骤。目前应用最为广泛的方法是离子交换色谱法，但这种方法所需时间长，且费用昂贵。Sartorius公司的Vivapure产品，为蛋白质的分离和纯化提供了全新的思路。离子交换层析是分离纯化蛋白最常用的方法，Vivapure也采用了这一原理，在很多蛋白质研究领域中可替代传统的HPLC和FPLC法，并克服了传统方法费时长、操作复杂、设备昂贵等不足。只需不到30分钟，就能完成蛋白样品的吸附、清洗和洗脱全过程，得到高纯度的蛋白质。

Vivapure centrifugal ion exchange membrane spin columns will dramatically change the way you isolate and purify proteins. Vivapure replaces time-consuming, tedious and expensive chromatographic methods for many protein applications. The rapid purification protocol based on Membrane Adsorbers allows the parallel separation of proteins with high yield in less than 20 minutes.

Ion exchange chromatography is among the most widely used methods for fractionation and purification of biological substances. Vivapure employs an innovative and powerful ion exchange membrane technology for separating proteins. The purification protocol involves only a few steps, making the isolation of your target protein from samples almost as simple as filtration.

Vivapure spin columns are available with strong or weak cation or anion charged membrane matrices. With these ion exchange membrane devices, protein binding, elution and consequent purification and concentration are made almost as simple as filtration.

The protein purified using the Vivapure spin column may be used in many downstream applications, e.g. sample fraction for 1D and 2D, SDS-PAGE, X-ray crystallization, NMR spectroscopy and related proteomics applications

Vivapure离子交换膜蛋白纯化浓缩器的典型性能

Vivapure离子交换膜蛋白纯化浓缩器可提供的膜类型

Vivapure离子交换膜蛋白纯化浓缩器的容量和规格

Vivapure离子交换膜蛋白纯化浓缩器的应用

• Fractionation of protein mixtures prior to 2D-PAGE
• Sample preparation prior to 1-D or 2-D PAGE
• Removal of endotoxins from monoclonal antibodies
• Preparation of heme moiety from heme containing protein for functional analysis
• Polishing of His-tagged proteins
• General protein purification
• Detergent removal from protein solutions
• Purification of antibodies from serum, ascites or tissue culture supernatant
• Establishing a purification/prepurification protocol for a given protein
• HPLC/FPLC sample preparation
• Purification of membrane-bound proteins

Vivapure离子交换膜蛋白纯化浓缩器的化学兼容性

Vivapure离子交换膜蛋白纯化浓缩器产品选择