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Parallab 350型全自动基因组工作站
Parallab 350 Fully Integrated Nanoliter Genomics Workstation
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TopSpot E Microarrayer
TopSpot E is a semi-automated entry level arrayer designed
for individual research projects and assay development.
Superior results are obtained in printing microarrays of
proteins and nucleic acids. |
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The core
of TopSpot technology is the micromachined print head. This
print head allows for 24 or 96 different liquid samples to
be loaded into separate reservoirs. The reservoirs are
spaced at standard 384 or 1536 microtiter plate format
facilitating easy filling by hand or automated pipetters.
Once loaded, hundreds of prints can be made without
reloading. Loaded samples are dispensed simultaneously at a
pitch of 500 µm with a frequency of about 1 array per
second. All print heads are reusable and easy to clean. |
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The
TopSpot E holds and positions the print head under a piezo
actuator. The slide to be printed is placed on a
motor-driven holder which automatically locates the slide
immediately beneath the print head. All printing steps are
controlled and tracked by a user friendly program, connected
via USB port. The TopSpot E system includes a 24 channel
print head, a print head cleaning station, control software
and accessories. The low cost, compact size and ease of use
make TopSpot E an ideal choice for individual labs. |
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System Specifications: |
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Slide
capacity |
1
slide, unlimited slides per print run |
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Printhead Reservoir pitch |
384
(24 Channels) or
1536 format (96 Channels) |
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Print
media |
Oligonucleotides, cDNA, Proteins, Antibodies, and Cells |
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Sample uptake volumes |
1 - 5
µl (24 Channels) or
1 - 3 µl (96 Channels) |
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Typical dispense volume |
1.0
nl |
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Typical spot diameter |
200
µm, depending on the substrate |
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Print
speed |
1
Hz/96 samples |
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Throughput |
approx. 60 slides per hour, depending on layout |
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Print
capacity |
up to
1,000 features per sample without refill |
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Physical Dimensions |
35 cm
x 18 cm x 30 cm |
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Weight |
approx. 10 Kg |
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TopSpot Technology
Parallabs offers a unique combination of microfluidic
and piezo technologies in the TopSpot/E system.
The microfluidic print head is composed of 3
layers. The glass upper reservoir layer is fixed to the
silicon fluid channel layer. The channels are of
identical length and volume capacity. They connect the
reservoirs with the glass print nozzles precisely
machined into the lower layer.
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1) Upper layer
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2) Miiddle layer
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3) Lower layer
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Click on the pictures to view a video of the print
head in action.
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Piezo actuated printing dispenses identical
drops from all channels simultaneously.
Click on this picture to view a video of droplet
dispensing |
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Accurate, reproducible dispensing of a wide
variety of samples. The TopSpot delivers common
laboratory liquids wiith CV of ejected volume <1%. |
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Tech
Note highlighting TopSpot technologies and printing of
protein and nucleic acid microarrays
Highly Parallel Dispensing of Chemical
and Biological Reagents
Click here for downloadable PDF copy |
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TopSpot Applications
Printing Protein Microarrays
TopSpot is a unique tool for printing protein
microarrays. No pins means no interaction with grooved
steel and no impact on coated slides. Zero
cross-contamination, zero carryover. Excellent
reproducibility.
Tech
Note:
Fast and Reliable Protein Microarray Production
by a New Drop-in-Drop Technique
Click here for downloadable PDF copy
Printing Nucleic Acid Microarrays
TopSpot has been used extensively for printing DNA
microarrays in low and medium density format.
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Example of over spotting PCR products on nylon
coated slides: |
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Tech
Note:
Highly Parallel Dispensing of Chemical
and Biological Reagents
Click here for downloadable PDF copy |
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在线产品样本(PDF格式) |
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在线产品样本(PDF格式) |
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上次更新时间：2008年09月08日
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