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GMO Analysis
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According to the European regulations on
genetically modified (GM) foods, a product is subject to
mandatory labeling if it consists of, or is derived from
genetically modified organisms (GMOs). The threshold for
labeling is 0.9% of each ingredient for EU-authorized GMOs.
This threshold is 0.5% for GMOs not authorized for marketing
in Europe but having successfully passed all safety assessments.
The new regulations relate to food, feed, flavorings, and
additives and require full traceability systems throughout
the entire production chain. Accurate and high-performance
tools are required for the quantification of the GM material
contained in all the different matrices from the seed to
the final product. Real-time PCR is the gold standard used
for GMO detection and quantification in foods. |
| Most of the GM crops currently available
are maize and soya. They account for more than 80% of the
total GMO-planted area worldwide. About 30,000 ingredients
today contain maize and soya in their formulation and are
affected by GMO traceability and labeling. |
| Bio-Rad iQ-Check GMO kits provide a simple
decision-making solution for manufacturers concerned by
maize and soya ingredient labeling. iQ-Check GMO are ready-to-use
kits based on real-time PCR. The kits enable reliable and
accurate detection and quantification of the level of GMO
presence in each sample: from raw material to processed
food. |
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iQ-Check GMO Detection and Quantification Principle
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According to European regulations, the genetically
modified organism (GMO) content is determined as a percentage
of the GM DNA present in the sample and the total maize
or soya DNA content. |
| The GM DNA is detected by the screening
of a special sequence, the promoter p35S, present in most
transformation events in GM crops. |
| iQ-Check screen GMO p35S/tNos kit detects,
in parallel with the p35S sequence, a terminal sequence,
tNos, present in most unauthorized GMOs on the market. |
| GM DNA is always detected and quantified
in the presence of an artificial sequence called the internal
control. This sequence is included in the amplification
mix provided in the kit and is used to assess the efficiency
of the amplification reaction. |
| Total maize and soya DNA content is determined
by the presence of a specific plant reference gene. |
| The quantification standards included in
the iQ-Check GMO quantification kits enable precise determination
of the GM DNA/total maize or soya DNA ratio. |
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iQ-Check GMO Detection and Quantification Principle
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Ready-to-use reagents for maximum reproducibility |
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Reliable detection of GMO presence |
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Accurate quantification of 20–20,000 DNA copies |
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No post-PCR operations |
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No post-PCR contamination risk |
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Minimal hands-on time |
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Flexibility to use different thermal cyclers |
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iQ-Check GMO Kit Contents
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iQ-Check Kits for Detection and Quantification
of the Level of GMOs
The kits contain all the reagents required to perform real-time
PCR analysis of the sample:
• Fluorescent probes
• Amplification mix containing Taq DNA polymerase, primers,
and internal inhibition control
• Positive and negative controls
• Ready-to-use quantification standards in all GMO quantification
kits
Reagents are provided in a ready-to-use format for 96 PCR
reactions. |
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iQ-Check GMO Kits
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产品编号 |
产品描述 |
| EasyPrep Extraction Apparatus |
| 357-8111 |
EasyPrep DNA Extraction System, dual-unit apparatus
for genomic DNA extraction and purification |
| iPure GMO Extraction Kit |
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357-8112 |
iPure GMO DNA Extraction Kit, ready-to-use reagent
kit for genomic DNA extraction and purification,
48 tests |
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GMO Extraction System
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A reliable genetically modified organism (GMO) testing
result is highly dependent on the quality of the DNA extracted
from a sample for analysis by real-time PCR. Food and plant
samples are extremely heterogeneous; they contain many different
compounds, such as fats and polysaccharides, which can lead
to suboptimal DNA extraction and processing. |
| The Bio-Rad DNA extraction system consistently
provides the user with high-quality DNA, free of any material
that could interfere with downstream applications such as
PCR, and is specifically suited to the treatment of GMO-related
matrices. |
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EasyPrep Extraction Apparatus
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The EasyPrep apparatus represents a technology that
responds to a rapid and standardized preparation of nucleic
acids while remaining truly simple to operate. The system
combines positive-pressure processing with advanced membrane
devices, adapted media, and intelligent chemistry. |
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iPure GMO Extraction Kit
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Suitable for use with the EasyPrep apparatus, the iPure
GMO extraction kit allows the rapid availability of PCR-ready
DNA for GMO detection. The kit is suitable for extraction
from all kinds of samples to be analyzed. |
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iPure GMO Extraction Kit
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Universal: one protocol for all matrices |
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Extraction of up to 1 g of sample |
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Reproducible high DNA quality for reliable PCR |
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Superior efficiency with complex samples |
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24 samples in parallel |
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Ready-to-use reagents |
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2.5-3 hour protocol |
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A single centrifugation step |
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Contents of the GMO Extraction System Kit
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Each kit contains all the reagents necessary
for 48 extractions:
• Anion exchange resin
• Solubilization, lysis, coating,
wash, and elution buffers
• Extraction columns
• Isopropanol
• Ethanol 70% |
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Bulletin_2697_GMO Testing.pdf |
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在线产品样本(PDF格式) |
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